- Research article
- Open Access
The zipper mechanism in phagocytosis: energetic requirements and variability in phagocytic cup shape
© Tollis et al; licensee BioMed Central Ltd. 2010
- Received: 7 July 2010
- Accepted: 8 November 2010
- Published: 8 November 2010
Phagocytosis is the fundamental cellular process by which eukaryotic cells bind and engulf particles by their cell membrane. Particle engulfment involves particle recognition by cell-surface receptors, signaling and remodeling of the actin cytoskeleton to guide the membrane around the particle in a zipper-like fashion. Despite the signaling complexity, phagocytosis also depends strongly on biophysical parameters, such as particle shape, and the need for actin-driven force generation remains poorly understood.
Here, we propose a novel, three-dimensional and stochastic biophysical model of phagocytosis, and study the engulfment of particles of various sizes and shapes, including spiral and rod-shaped particles reminiscent of bacteria. Highly curved shapes are not taken up, in line with recent experimental results. Furthermore, we surprisingly find that even without actin-driven force generation, engulfment proceeds in a large regime of parameter values, albeit more slowly and with highly variable phagocytic cups. We experimentally confirm these predictions using fibroblasts, transfected with immunoreceptor FcγRIIa for engulfment of immunoglobulin G-opsonized particles. Specifically, we compare the wild-type receptor with a mutant receptor, unable to signal to the actin cytoskeleton. Based on the reconstruction of phagocytic cups from imaging data, we indeed show that cells are able to engulf small particles even without support from biological actin-driven processes.
This suggests that biochemical pathways render the evolutionary ancient process of phagocytic highly robust, allowing cells to engulf even very large particles. The particle-shape dependence of phagocytosis makes a systematic investigation of host-pathogen interactions and an efficient design of a vehicle for drug delivery possible.
- Actin Polymerization
- Fluorescence Recovery After Photobleaching
- Phagocytic Uptake
- Membrane Fluctuation
- Zipper Mechanism
Phagocytosis is the ancient, evolutionarily conserved process by which eukaryotic cells bind, engulf, and destroy particles and cells larger than 0.5 μ m in diameter [1–3]. The importance of phagocytosis is derived from its two main functions: (1) a feeding mechanism in single-cell organisms , and (2) the clearance of pathogens, apoptotic and senescent cells from our body by immune cells [5, 6]. As part of our immune defense, phagocytosis is mainly performed by professional phagocytes, including macrophages, neutrophils, and dendritic cells. Initiation of phagocytosis occurs with recognition of the target particle either directly or via an opsonising molecule. For instance the Fc portion of immunoglobulin G (IgG) is recognized by the cell-surface receptor FcγRIIa [7, 8]. Ligand-receptor binding triggers intracellular signaling [3, 8, 9], resulting in remodeling of the actin cytoskeleton [10, 11] and coherent growth of cell membrane around the particle to form the phagocytic cup [1, 2]. Eventually, the leading edge of the growing cup closes, and a membrane vesicle enclosing the particle (phagosome) moves inside the cell. Subsequently, the phagosome fuses with vesicles containing enzymes [12, 13], acids , and oxygen radicals [15, 16] to destroy the particle.
The biochemical pathways involved in phagocytosis are complex. Dozens of cell-surface receptors contribute to the recognition of a large variety of ligand molecules and subsequent particle engulfment [3, 8, 17]. The Fcγ receptor (FcγR)  and complement receptor 3 (CR3) of the integrin receptor family  are the most widely studied and understood receptors involved in phagocytosis. FcγR-mediated phagocytosis proceeds through membrane protrusions and leads to thin cups [20, 21], whereas in CR3-mediated phagocytosis, particles appear to sink into the cell [22, 23]. Spreading of the cell membrane over the particle involves actin-driven cell-shape changes similar to the processes involved in cell migration and adhesion [7, 24–27]. Specifically for FcγR, binding to an IgG-opsonized particle results in receptor clustering and recruitment of small GTPases of the Rho family, which, via proteins of the WASP family, activate the Arp2/3 complex [1, 3]. The latter promotes branching of actin filaments, leading to an increase in the number of uncapped ends and to an isotropic growth of the actin network [8, 28]. Additionally, the phagocytic cup has been shown to be enriched in gelsolin [29–31], coronin , and other regulators of actin polymerization. All in all, this complex signaling pathway involves 100-1000 different types of molecules [3, 32], rendering mathematical modeling at the molecular level impossible.
Despite the huge biochemical complexity, the engulfment process shows a strong dependence on simple biophysical parameters. First, it relies on the availability of extra membrane at the phagocytic cup [33, 34], provided by delivery of membrane vesicles  or unwrinkling of membrane folds [36, 37]. Second, completion of phagocytic uptake depends on the shape of the particle and, interestingly, on the initial orientation of the particle on the cell surface [20, 38]. For instance, experiments demonstrate that elongated spheroid polystyrene particles coated with IgG are more efficiently engulfed when presented to the phagocyte with their tip first. Third, a recent study by one of the authors demonstrates that the biophysical requirements for phagocytosis lead to either complete phagocytosis or stalled cups due to the presence of a mechanical bottleneck . Interestingly, the same study shows that engulfment appears to even proceed in cells treated with (modest amounts of) cytochalasin D, an inhibitor of actin polymerization, indicating that biochemical pathways may not always be necessary for this initial stage of phagocytosis.
Recent experiments provide new insights into the biophysical mechanism for driving the membrane around the particle, suggesting a ratchet-type mechanism. Once started, phagocytosis progresses unidirectionally and irreversibly . This irreversible membrane progression is further supported by the loss of lipid and protein mobility at the phagocytic cup, observed using fluorescence recovery after photobleaching (FRAP) . While several models proposed mechanisms of force generation by actin polymerization (see  and references therein), recent experiments based on fluorescent speckle microscopy demonstrate that actin does not directly push the membrane outwards. Instead, by filling gaps provided by membrane fluctuations (or other types of membrane movement), actin polymerization prevents the membrane from moving backwards like a ratchet . The relevance of such Brownian ratchets in biology has previously been emphasized [49, 50]. The question is if a ratchet mechanism, together with energetic restrictions in membrane bending and stretching, can naturally lead to phagocytic uptake and account for the shape-dependence of phagocytosis.
In this work, we propose a ratchet-like biophysical model for the zipper mechanism. This model differs from previous works in that it is, to our knowledge, the first fully three-dimensional stochastic model of phagocytic engulfment. Specifically, thermal membrane fluctuations, assumed to play a major role in our model, provide the energy source to locally deform the membrane and to build further ligand-receptor bonds for zippering the membrane around the particle. Actin polymerization makes ligand-receptor bonds effectively irreversible, i.e. reinforced and stabilized for a significant amount of time. To investigate the role of actin, we compare cup progression for the regular active zipper with a passive zipper model in which ligand-receptor binding remains specific and strong but reversible due to the absence of actin polymerization.
Interestingly, we find that the passive zipper also leads to engulfment of small particles, rendering phagocytosis highly robust. However, such passive engulfment is generally slower and produces much more variable phagocytic cups than the active zipper. Furthermore, our computer simulations lead to successful phagocytic engulfment in a broad range of parameters values, including different particle sizes. For non-spherical particles, completion of engulfment depends strongly on particle shape and orientation. Our model further predicts that cup shape invariably depends on membrane biophysical parameters, in particular surface tension and cell-volume constraint.
To test the predicted difference between the active and passive zippers, we experimentally implement the two different types of zippers using COS-7 fibroblasts which, after transfection with GFP-tagged Fcγ receptor, phagocytoze IgG-coated polystyrene particles. Specifically, we performed phagocytic assays under three different conditions: (1) cells expressing wild-type FcγR for the active zipper (WT-FcγR), (2) cells expressing a signaling-dead mutant receptor (Y282F/Y298F-FcγR), which specifically binds IgG ligand but is unable to signal to the actin cytoskeleton [39, 51, 52], and (3) cells expressing WT-FcγR and treated with cytochalasin D (WT-FcγR+CytoD). The last two conditions represent two versions of the passive zipper due to the absence of actin polymerization in phagocytic cups. To compare with our model, we systematically analyze confocal microscopy images, and quantitatively estimate cup variability for the three different conditions using small (1.5 μm radius) and large (3 μm radius) particles. Consistently with our simulations, phagocytic cups develop more slowly and are significantly more variable in the absence of actin polymerization. Our results provide new insights into the robustness of phagocytosis, as well as the role of bacterial cell shape in host-pathogen interactions.
Ratchet model for the zipper mechanism
Our model is based on the following experimental observations. Engulfment of quasi-spherical particles by neutrophils progresses continuously without significant pause or reversal, indicating that ligand-receptor binding is essentially irreversible . This irreversibility is further supported by FRAP and single-molecule experiments, which show that lipids and proteins in phagocytic cups, as well as ligand-bound Fc-receptors are immobilized in an actin-dependent fashion [47, 53]. Additional support for the notion of irreversible uptake was recently determined in a related context . Fluorescent speckle microscopy of actin flow and image analysis during cell migration show that the membrane at the leading edge protrudes first, followed by actin polymerization to fill the gap between the membrane and the actin cortex. Such actin polymerization is mainly restricted to the leading edge due to signaling by receptors and/or localization of small GTPases of the Rho family . The role of actin polymerization in phagocytosis is hence to stabilize ligand-receptor bonds and to rectify membrane movements in a ratchet-like fashion, leading to unidirectional movement of the leading edge of the engulfing cell.
Figure 1A introduces the general concept of the zipper mechanism in phagocytosis, and Figure 1B summarizes our ratchet model for this mechanism. The cell membrane and the actin cortex are described by a Helfrich-type energy function , including contributions from ligand-receptor binding, membrane bending and stretching, as well as a cell-volume constraint. Chosen membrane parameters effectively describe the cell plasma membrane with its underlying actin cortex. The model was implemented using finite-temperature Monte Carlo simulations of the discretized cell membrane (see Methods for details). Briefly, the algorithm proposes random, thermally generated membrane fluctuations (trial moves), which are either accepted or rejected depending on the change in total energy during the move. When a random membrane fluctuation brings the cell membrane in contact with the particle, this fluctuation is likely accepted due to energetically favorable ligand-receptor binding. Once accepted, this fluctuation is made irreversible as a result of signaling and actin polymerization. In contrast, a membrane fluctuation far away from the particle is less likely to be accepted. Even if accepted, the fluctuation is not made irreversible and hence may retract at a later time (see Additional file 1, Figure S1). Hence, in our model the actin network only supports membrane fluctuations which lead to progression of the engulfing zipper as a result of signaling.
Dependence of phagocytic cup shape on membrane biophysical parameters
Active versus passive uptake and the role of actin
Although phagocytosis generally involves active processes such as actin polymerization in the cup (active zipper) , recent reports indicate that phagocytosis may still work in an actin-independent manner. Indeed, phagocytic uptake was observed despite treating phagocytes with (modest amounts of) cytochalasin D . Hence, ligand-receptor binding may be sufficient in guiding the cell membrane around the particle under certain conditions (passive zipper). To investigate the energetic requirements of the zipper mechanism, we implemented simulations of the passive zipper. In these simulations, ligand-receptor bonds are not stabilized by actin polymerization and can unbind at later times, i.e. remain reversible. Hence, engulfment may still progress if the energetic cost of stretching and deforming the membrane is offset by the ligand-receptor binding energy in the presence of thermal membrane fluctuations.
Figure 3 (right) compares time courses of the membrane energy and progression of uptake for the active and passive zippers. We found that in both cases, membrane energy decreases rapidly at the very beginning of the uptake process due to energetically favorable ligand-receptor binding without large, energetically unfavorable deformations of the cell membrane. After this short initial period, the total energy increases with simulation time. This increase is much more pronounced for the active zipper, which stabilizes energetically unfavorable random membrane fluctuations by actin polymerization. In contrast, for the passive zipper such high-energy membrane deformation may not last over time. The slower increase in energy for the passive zipper correlates with a slower engulfment. For the simulations shown, engulfment for the active zipper is approximately twice to three times as fast as for the passive zipper, although the latter eventually engulfs the particle. However, the difference between active and passive engulfment depends on biophysical parameters, and may be reduced for particular choices of the parameters values, e.g. lower surface tension and/or stronger ligand-receptor binding.
Particle size matters for passive, not for active zipper
Experiments show that phagocytosis is relatively insensitive to particle size . Using our model for the active zipper, we simulated engulfment of spherical particles with different radii ranging from 1.2 to 3.8 μ m. Figure 2C shows that engulfment progresses normally for small and large particles. Hence the active zipper mechanism is sufficiently robust to allow engulfment of differently sized particles using the same set of biophysical parameters, although engulfment of large particles requires more time. Noticeably, large particles (in general, with radius larger than 2.5 μ m) were taken up via more regular phagocytic cups than small particles (with radius 1.5 μ m or smaller), indicating that active processes may be required for engulfment of large particles. To confirm this observation we have simulated engulfment of large 3 μ m-radius particles by both the active and the passive zipper, shown in Additional file 1, Figure S5. While the active zipper resulted in complete uptake of the particle, the passive zipper only engulfed a few percent of the particle's surface area. Thus the difference in phagocytic efficiency between the two zipper types was exacerbated for large particles, reflecting the importance of actin polymerization for engulfment of large particles.
Experimental test of model predictions
To confirm that our results are independent of the specifics of the analysis method used, we also analyzed phagocytic cups with an alternative, albeit less accurate, method (see Additional file 1, Figure S9). This method consists of determining the distribution of FcγR-GFP fluorescence intensity around the particle at its equator plane, restricting the analysis to approximately half taken up particles (30-70%). The standard deviation of this distribution provides an alternative measure of the cup variability. We arrived at the same conclusion, confirming our result of the difference in cup variability (see Additional file 1, Figure S10). Finally, note that temperature-induced synchronization is imperfect and may lead to variability in cup growth . However, our measures of the variability in cup shape are independent of such an effect since we include all time points together in the analysis (except for plots showing the time dependence of engulfment).
Our experiments further show that cup shape ranges from regular to variable for all three experimental conditions, but that the frequency of different cup shapes depends on the condition. Figure 5C plots the repartition of phagocytic cups for different conditions into both regular and variable cups. Note that a cup was identified as regular if its variability was below the variability averaged over all experimental conditions. In contrast, a cup was identified as variable if its variability was above the overall average. This plot shows that in our experiments a regular cup is most likely produced by a cell expressing wild-type receptor, whereas a variable cup is most likely to be produced by a cytochalasin-D treated cell. Hence, the cup shape has universal features independent of biochemical details. Examples of a regular and a variable cup are provided in Figures 4E and 4F, respectively. Both cups were taken from a cytochalasin-D treated cell, confirming that a regular cup can occur under any of our experimental conditions.
Note that for the time points measured, the average uptake does not exceed 50%, even for WT FcγR. This is caused by the fact that some particles are not engulfed irrespective of the condition  (see Additional file 1, Figure S6), reducing the average percentage of engulfment. The proportion of almost completely engulfed particles (with engulfed surface area larger than 70%) beyond 6 minutes is represented in the inset of Figure 6A, showing that completion of engulfment is possible even for cells without actin polymerization. Note that long phagocytic assays (12, 14, and 45 minutes) were performed for cytochalasin-D treated cells only, explaining why the difference in complete engulfment with cells expressing the wild-type receptor is smaller than one may expect.
From these results, we conclude that functional actin polymerization is required for fast and regular engulfment in phagocytosis. Nevertheless, in line with our model predictions, cells showing deficient actin polymerization at cups are still able to uptake small particles, although more slowly and with more variable cups.
Active zipper reproduces particle-shape dependence of phagocytosis
Previously published experiments show that phagocytosis depends strongly on particle shape. In particular, elongated particles (similar to rod-shaped bacteria) are only taken up when presented to the phagocyte with their tip first [20, 38]. To test the particle-shape dependence of phagocytosis we conducted simulations of the active zipper using particles of different shapes while varying the initial orientation of the particle on the cell surface. Figure 2D shows the uptake of a prolate spheroid for two different orientations on the cell membrane after the same elapsed simulation time. In accordance with experimental observations [20, 38], uptake is more advanced for the spheroid particle engulfed with its tip first (about 80% engulfed surface area) than for the particle attached along its major axis (about 50% engulfed in the same amount of time). This suggests a strong inhibitory effect of high local curvature on uptake. In our model this is readily attributed to the energetic cost of bending the membrane around the two highly curved ends of an elongated particle placed horizontally on the cell membrane. In line with this explanation and experiments , the spiral-shaped particle in Figure 2E is not engulfed after a time duration sufficient for the engulfment of large spherical particle of twice its volume. Hence, our simulations demonstrate that particle shape and orientation are indeed important biophysical parameters for phagocytosis.
In this work, we studied the biophysical requirements of the zipper mechanism, in particular the role of receptor-induced actin polymerization, and the effect of particle shape on uptake. In our model, the underlying biophysical mechanism of the zipper is an actin-driven thermal ratchet, which renders random membrane fluctuations irreversible close to the particle (Figure 1). This mechanism is supported by several recent experiments [37, 47, 54]. Previously, such Brownian ratchet models were successfully applied to explain force generation by actin polymerization and motility of the pathogen Listeria in hosts cells . Our fully stochastic simulations can address for the first time the variability in particle uptake, recently noticed in phagocytic cup growth  and completion of endocytosis . Implementation of our model in simulations led indeed to phagocytic engulfment for a broad range of values of membrane parameters (Figure 2), indicating exquisit robustness of the phagocytic process. However, phagocytic cup shape depends on parameter values, specifically on the ratio between surface tension and cell-volume constraint, as well as on the kinetics of engulfment. Cells with low surface tension and/or tight volume constraint develop thin cups (Figure 2A, left), characteristic of FcγR-mediated phagocytosis [4, 22]. In contrast, cells with high surface tension produce broad cups (Figure 2A, right). The latter cup shape is more reminiscent of CR3-mediated phagocytosis, although for this type of phagocytosis particles are believed to sink into cells without protrusive cups [22, 23].
Using our model we were able to address the question whether the zipper mechanism requires an active driving force, such as provided by actin polymerization. For this purpose, we compared the regular active zipper with a passive version of the zipper. In the passive zipper, ligand-receptor bonds are as strong as for the active zipper (based on experimental observation ) but are reversible, i.e. are not supported by actin polymerization. We demonstrated that the passive zipper also leads to engulfment of small particles (of radius 1.5 μ m), although cup progression is slower and more variable (see Figure 3). Our active zipper can also reproduce the independence of uptake on particle size, in line with experimental observations . In contrast, large particles (of radius 3 μ m) are poorly phagocytozed by the passive zipper. We subsequently confirmed these predictions with experiments by transfecting COS-7 fibroblasts with wild-type FcγR and signaling-dead mutant Y282F/Y298F FcγR (see Figures 5 and 6). While the wild-type receptor represents the active zipper, the passive zipper is implemented through the use of signaling-dead mutant receptor or treatment with cytochalasin D. Both prevent actin polymerization in the cups. Our study may indicate that ancient forms of phagocytosis were driven by physical (passive) principles, and only later in evolution biochemical regulatory pathways were added for further support and robustness. Passive phagocytosis may also become important when energy sources are scarce.
Despite the robustness of phagocytosis to particle size, there appears to be a mechanical bottleneck around half-engulfment, recently observed by imaging  and also predicted by our model. For slight variations in some of the parameters, our simulations of the active zipper produce either complete or significantly incomplete uptake (see Additional file 1, Figure S6). Indeed, when the cup grows, deforming the membrane costs more and more energy per surface area engulfed. Beyond half-engulfment, the surface tension energy is twice as high as at the beginning of engulfment due to the membrane folding back onto itself. If this energy cannot be provided by the zipper, then cup progression stalls and the particle remains incompletely engulfed. Alternatively, the experimental data on incomplete particle uptake may be the result of particle attachment on cell-membrane areas, unable to phagocytose due to other reasons, such as unfavorable local cell-surface curvature, proximity to cell edge or nucleus, or missing proteins, lipids, and smaller molecules belonging to key signaling pathways. Further studies will be required, ideally using live-cell imaging to avoid the need for conserving the cell's internal structures by fixation .
Our model for the zipper mechanism can also explain the strong particle-shape dependence observed in phagocytosis. Experiments show that an elongated spheroid is rapidly engulfed if the particle attaches to the cell membrane with its tip, but not if the particle attaches along its major axis [20, 38]. Furthermore, spiral-shaped particles are not phagocytozed . The strong particle-shape dependence of phagocytosis is likely of biological relevance. On the one hand, it may increase the rate of infection of host cells by pathogenic bacteria. Indeed, recent experiments show that Mycobacteria tuberculosis and marinum are efficiently taken up and later released for spreading of the infection with the bacteria's tip first . On the other hand, the highly curved shapes of some bacteria, e.g. the spiral-shaped Helicobacter and Campylobacter species, may prevent their uptake by macrophages , although injection of effector proteins can also be used by pathogens to hijack the immune or host cell's phagocytic response [58, 59]. Furthermore, the particle-shape dependence of phagocytosis may be exploited to improve drug delivery by enclosing active drugs in particles, whose shape prevents uptake and destruction by macrophages [38, 60].
While our biophysical model for the zipper mechanism is readily accessible for analysis and interpretation, the small number of model parameters makes a direct comparison with measured parameter values difficult. First, our membrane parameters such as surface tension and bending stiffness are an order of magnitude smaller than reported bulk membrane parameters (see Methods). This reduction is not surprising as cells regulate these parameters locally for efficient uptake [61–63]. Such regulation may include lowering of the surface tension by local membrane delivery through vesicles [35, 64, 65] and unfolding of membrane wrinkles , as well as changes in the lipid and protein composition in the phagocytic cup . Second, our description of ligand-receptor interaction assumes that ligand and receptor distributions are continuous and homogeneous, while experiments indicate the formation of receptor micro-clusters , possibly as part of lipid rafts . Third, our mechanism for actin polymerization based on membrane fluctuations neglects the role of the motor proteins (myosin I and II), whose role in membrane deformations has been established [67, 68]. Consequently, our simulations describe well the dynamics of engulfment during the first two thirds of the uptake. At later stages of uptake, experiments show that phagocytic cups close rapidly with a thin membrane protrusion [4, 69], while our simulations show slow cup closure. However, taking into account myosin-driven contraction is beyond the scope of the current work.
Our model may also be applicable to other biological systems in which a zipper-like mechanism is involved. One such example is sporulation of Bacillus subtilis during starvation. After asymmetric cell division, the larger mother cell engulfs the smaller forespore for spore maturation. Interestingly, the mother cell even engulfs the forespore when the cell wall is artificially removed. This process occurs in a fast, zipper-like fashion without known sources of energy . Importantly, forespore engulfment is subject to high variation. About 60% of the cells successfully complete forespore engulfment, while 40% do not at all, similar to the observation of the mechanical bottleneck in phagocytosis. Other examples of engulfment may not be driven by a zipper mechanism. For instance, the penetration of red blood cells by the malaria Plasmodium merozoite is driven by an elaborate actin machinery of the particle (the parasite), which devotes all its resources to wrap the cell membrane around itself. In stark contrast to phagocytosis, the engulfing host cell is completely passive .
Cell membrane and particle models
where the integral is performed over the cell-membrane area. Effectively, is given by the product of the individual ligand-receptor binding energy and the density of ligand-receptor bonds, divided by the density of vertices on the model membrane (see below). The width of the square potential R0 is chosen to be very small compared to the other length-scales of the model R0 < 0.1 R and does not influence the results.
Simulations of phagocytic engulfment were implemented by discretizing the cell and particle surfaces using the Surface Evolver software . This software is designed to perform energy minimization on flexible surfaces, and is freely available from http://www.susqu.edu/facstaff/b/brakke/evolver. The software includes a built-in programming language, which we used to implement a Monte Carlo algorithm (see below). The cell membrane is approximated by a finite number of vertices, used to create a triangular mesh. The software computes the local energy density at each vertex and sums up the energy contributions from all the surface elements to obtain the total free energy Eq. 1.
Main model parameters
Our model uses four tunable biophysical parameters. Unless otherwise specified, we have used the set of Standard Parameters (SP), chosen according to experimental measurements [37, 42, 61, 74] when possible (see Supplementary Information, section 1), but ultimately to produce realistic cup shapes (see Figure 2). This set of parameters includes: the cell membrane bending rigidity κ b and surface tension σ, respectively set to 1.3 × 10-2 pNμ m and 6.2 × 10-6 mNm-1, i.e. slightly below the experimentally measured values since local changes in chemical composition of the cups membrane may reduce these parameters [1, 8, 36, 75–77]. The third parameter is the total binding energy density ϵ = 58.5 pNμ m-1. This value was estimated from measurements of the individual FcγR-IgG binding free energy ΔF LR ≈ 20 k B T [78–81], the average density d LR = 270 - 435 μ m-2 of IgG-FcγR bonds , and the fact that in response to diffusion and trapping or signaling, receptors may cluster at the cup. Finally, the local constraint on cell-volume has been chosen κ P = 2.56 × 10-5 pNμ m-5 to allow 20 percent volume variation in line with observation [83, 84].
Monte Carlo simulations
The Surface Evolver was only used to obtain a triangular mesh (vertices connected by edges) of the cell membrane, and to resample the membrane as the uptake progresses. The cell-membrane evolution was implemented using finite-temperature Monte Carlo Metropolis simulations [85, 86]. Details of the simulation can be found in the Supplementary Information, section 2. Briefly, our algorithm calculates the total energy of the initial membrane configuration, then randomly selects a point to be the center of a membrane fluctuation, and a random direction and lateral extension of the fluctuation. Subsequently, the energy of the new membrane configuration is calculated, and compared to the initial energy. If the membrane fluctuation decreased the energy, the trial fluctuation is accepted and the procedure is reiterated starting from the new membrane configuration. To the contrary, if the membrane energy increased with the trial fluctuation, the latter may be rejected with some probability depending on the configuration's energy difference. In this case, a new fluctuation is attempted from the initial configuration. Between trial fluctuations, the cell-membrane vertices are examined. For the active zipper, the vertices within the closed neighborhood of the particle are immobilized for the remainder of the simulation. For the passive zipper, every membrane fluctuation may be reversed at a later time.
Additional simulation parameters
(1) The amplitude of a membrane fluctuation. This parameter is set to 0.5 R0 in all the simulations. (2) The mesh size, describing the maximal distance between two neighboring vertices. This parameter is set to R0. (3) Minimal width of a membrane fluctuation. To ensure that a minimum number of vertices is involved in each fluctuation, this parameter is set to 4 R0. (4) Mesh refinement range. Our simulation script automatically refines the mesh locally around a previously immobilized vertex within this range. This parameter is set to the particle radius R, leading to reasonably smooth cup shapes in a reasonably short calculation time (1-2 days for complete uptake using a Intel(R) Core(TM)2 Quad CPU working at 2.50 GHz and run by the RedHat EL5 linux distribution with 4 GB RAM).
Cells, plasmids and antibodies
COS-7 fibroblast cells were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% foetal bovine serum (FBS) and penicillin/streptomycin (Invitrogen). cDNAs encoding wild-type and Y282F/Y298F human FcγRIIa from pRK5-FcγRIIa  and pRK5-Y282F/Y298F-FcγRIIa  were subcloned into pEGFP-N1 (Clontech) using primers 5'-ggtccaactgcacctcggt-3' and 5'-ccccccgaattctgttattact gttgacatggtc-3'. The cytoplasmic tail truncation mutant 239-FcγRIIa was generated from the pRK5-FcγRIIa template using primers 5'-ggtccaactgcacctcggt-3' and 5'-gggggggaattctcctgcagtagatcaagg ccact-3'. Rabbit anti-bovine serum albumin (BSA) serum was purchased from Sigma-Aldrich. Alexa-conjugated secondary antibodies and phalloidin were purchased from Invitrogen.
Transfection and phagocytic challenge
COS-7 cells were transfected with GFP-tagged FcγRIIa constructs using an Amaxa Nucleofector and Nucleofector cell line kit R following the manufacturer instructions. For phagocytosis assays, transfected cells were seeded onto glass coverslips in 24-well plates at a density of 15,000 cells/coverslip and incubated at 37°C for 72 h. 1 hour before commencement of phagocytosis assays, cells were incubated for 1 hour at 37°C with serum-free DMEM plus 10 mM Hepes (Invitrogen). 1.5 μ m- and 3 μ m-radius latex-polystyrene particles (Sigma-Aldrich) were opsonized by first incubating overnight at 4°C with 3% BSA fraction V in PBS (Sigma-Aldrich) followed by incubation with 1:100 dilution of rabbit anti-BSA in PBS for 1 hour at room temperature. Particles were re-suspended in ice-cold serum-free DMEM plus 10 mM Hepes at a concentration of 1.5 × 106 particles/ml and 500 μ l added to each coverslip. Plates were incubated on ice for 10 minutes to allow binding of particles. Medium was then replaced with pre-warmed serum-free DMEM plus 10 mM Hepes and plates were incubated at 37°C, then processed for scoring or microscopy as described below. Experiments carried out on cells treated with cytochalasin D were conducted as above with the addition of a 20 min pre-incubation step with 0.2 μ M cytochalasin D in serum-free DMEM plus 10 mM Hepes at 37°C immediately before incubation of cells with opsonized particles. This concentration of cytochalasin D was included in all further incubation steps.
Imaging of phagocytic cups
Plates were placed on ice after a 20 minute incubation at 37°C and medium replaced with a 1:500 dilution of anti-rabbit Alexa 488 in 3% BSA/PBS at 4°C for 5 min. Cells were fixed after incubation at 37°C for the appropriate amount of time with ice-cold 4% paraformaldehyde/PBS, permeabilised and labelled with goat anti-rabbit Alexa 633, and phalloidin Alexa 555 for visualizing F-actin at room temperature for 30 min. Z-series image stacks were acquired on a Zeiss LSM-510 confocal microscope using a step size of 0.4 μ m.
Image analysis of phagocytic cups
Two fluorescence channels (IgG, FcγR-GFP) were acquired and analyzed using MATLAB (MathWorks). During the acquisition process, the FcγR-GFP fluorescence intensity was set to zero at any pixel where the IgG intensity is null. The fluorescence intensity distribution of IgG was used to determine the coordinates of the centers of particles with their corresponding radii, using an automated search based on the Hough transform , available online at http://www.mathworks.com/matlabcentral/fileexchange/4985 within each 2D image. The percentage of engulfed particle surface area was calculated by comparing the local FcγR-GFP (cell membrane) and IgG (particle surface) intensity distributions within a sphere S0 of radius 4R/3 whose center coincides with the particle center. Two methods were used to quantify the variability of the cups. The method used in the main text cuts the three-dimensional image (and hence the circular projections of particles within each imaging plane) in twenty-four angular segments, and finds the highest plane in which FcγR-GFP fluorescence intensity is detected in the immediate neighborhood of the particle for each segment (see Figure 4). This analysis produces a distribution of membrane height versus angular segment index, for which we compute the average and the mean-square deviation. The average height reached by cell membrane is roughly proportional to the surface engulfed (see Additional file 1, Figure S10), and the mean-square deviation divided by the square root of the average height quantifies the variability of the phagocytic cup, excluding a trivial size dependence on the variability. The less accurate alternative method determines the angular distribution of FcγR-GFP fluorescence intensity within the particle's equator plane in the particle's immediate neighborhood, keeping only particles whose uptake level comprises between 30 and 70% (roughly half-engulfed particles). Specifically, this region is cut into twenty-four identical angular segments, for which the total FcγR fluorescence intensity is calculated. Then the average intensity per segment is calculated, as well as the standard deviation. The higher the standard deviation, the more variable the cup.
The FcγRIIa receptor is referenced in protein database Genbank http://www.ncbi.nlm.nih.gov/Genbank under the accession number CAA01563. Signaling-dead mutant receptor Y282F/Y298F-FcγRIIa is obtained by replacing tyrosines (Y) with phenylalanines (F) at positions 282 and 298.
This paper is dedicated to the memory of Emmanuelle Caron, who tragically passed away in 2009. We acknowledge Micah Dembo and Günther Gerisch for helpful discussions, and Ken Brakke for help with the Surface Evolver software. We thank Vania Braga, Tony Magee and Brian Robertson for careful reading of the manuscript, and Suhail Islam for computational support. All authors would like to acknowledge funding from the Center for Integrative Systems Biology at Imperial College (CISBIC). RGE was additionally supported by the Biotechnology and Biological Sciences Research Council grant BB/G000131/1.
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